Eggs are thoroughly disinfected in a hypochlorite solution during processing. Inventory is periodically tested, stored in a temperature-controlled (40 degrees F) warehouse, and packaged on demand.
Storing Brine Shrimp Eggs:
First of all, you need to start with healthy, properly stored eggs. All brine shrimp eggs need to be stored as follows:
in a tightly sealed container;
free from moisture; and
in a cool environment at or below 50°F. (Refrigeration is ideal for short term storage, i.e., less than three to four weeks; for longer term storage, eggs are best kept at or below freezing.)
We recommend that upon receiving your eggs, dividing them into an amount that will be consumed within three to four weeks and storing this amount in a tightly sealed container in the refrigerator; the remainder should be stored, also in a tightly sealed container, in the freezer. Keep in mind that freezing can lower metabolic activity and delay hatch-out. We suggest removing egg from the freezer one day in advance of using it.
The above storage guidelines apply to all brine shrimp eggs, whether in opened or unopened tins.
Hatching Environment Follow these guidelines for the best results:
25 parts per thousand (ppt) salt solution, or approximately 1 and 2/3 tablespoons of salt per quart (or liter) of water. This equates to around 1.018 specific gravity as measured with a hydrometer. Be sure to use marine salt or solar salt.
Proper pH is important in hatching brine shrimp. A starting pH of 8.0 or higher is recommended. In areas where the water pH is below 7, Epson salt or magnesium sulfate can be added at the rate of 1/2 teaspoon per quart of solution to buffer the hatching solution.
Optimum water temperature for a 24-hour complete hatch is 80-82°F or 26-28°C. Lowering the temperature would result in a longer hatching time. Do not exceed 30°C.
Illumination is necessary to trigger the hatching mechanism within the embryo during the first few hours of incubation. Maintaining a light source during the entire incubation period is recommended to obtain optimum hatch results and for temperature control.
Constant aeration is necessary to keep cysts in suspension and to provide sufficient oxygen levels for the cysts to hatch. A minimum of 3 parts per million dissolved oxygen during the incubation is recommended. Strong aeration should not damage or hurt the brine shrimp cysts or nauplii.
1 gram per liter or quart or approximately 1/2 level teaspoon of cysts per quart is recommended. A higher stocking density will result in a lower hatch percentage.
Flat-bottom hatching vessels should be avoided. Cone or “V” bottomed containers are best to insure that the cysts remain in suspension during hatching. Be sure to thoroughly wash the hatching cone with a light chlorine solution, rinse, and allow to air-dry between uses. Avoid soap. Soap will leave a slight residue which will foam from aeration during hatching and leave cysts stranded above the water level.
Generally, the optimum incubation time is 24 hours. Egg which has been properly stored for more than 2-3 months may require additional incubation time — up to 30-36 hours. Oftentimes, eggs will hatch in as few as 18 hours. If a smaller size nauplii (Instar I) is desired, a harvest time of 18 hours is recommended.
Helpful Hint: Brine shrimp egg is sometimes very buoyant. In order to maximize the hatching percentage, it is sometimes helpful to swirl the water inside the hatching container with your finger once or twice at intervals in the first 4 to 6 hours of incubation in order to knock down eggs that have been stranded on the side of the container above the water-line. After about 6 hours, the eggs are usually well-hydrated and will stay in the water column.